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The YSI 251398Y DPD Free Chlorine Reagent Powder is designed for accurate measurement of free chlorine in water samples using a compatible colorimeter. Each pack contains 100 individual powder packs, with each pack formulated for a 5ml sample volume and capable of measuring free chlorine in the range of 0 to 3.5 mg/L.
This reagent utilizes the DPD (N,N-Diethyl-p-phenylenediamine) method, which reacts with free chlorine to produce a magenta color, the intensity of which is proportional to the chlorine concentration and measured by a colorimeter.
Informações de segurança
Always handle chemical reagents with care. Read the Safety Data Sheet (SDS) for this product before use. Wear appropriate personal protective equipment (PPE), including safety glasses and gloves, when handling the reagent powder. Avoid ingestion and contact with skin or eyes. In case of contact, rinse thoroughly with water and seek medical attention if irritation persists.
Configuração e Preparação
Before beginning any measurements, ensure you have the following:
- YSI 251398Y DPD Free Chlorine Reagent Powder pack
- Compatible colorimeter or spectrophotometer
- limparample cells/vials
- Deionized or distilled water for blanks and dilutions (if necessary)
- Água sample a ser testado
- Temporizador
Passos de preparação:
- Ensure all glassware and sample cells are clean and free from contamination. Rinse with deionized water before use.
- Calibrate your colorimeter according to its specific instruction manual before use.
Instruções de operação
Follow these steps to accurately measure free chlorine using the YSI 251398Y DPD Free Chlorine Reagent Powder:
- SampLe Coleção: Colete água doceample in a clean sample cell. The required volume is 5ml. Ensure the sample is representative of the water being tested.
- Adicionar reagente: Carefully tear open one YSI 251398Y DPD Free Chlorine Reagent Powder pack. Add the entire contents of the powder pack to the 5ml water sample no sample célula.
- Mistura: Tampe o sample cell and invert several times to thoroughly mix the reagent with the sample. Ensure all powder dissolves completely. A magenta color will develop if free chlorine is present.
- Tempo de reação: Allow a reaction time of 1 minute for the color to fully develop. Do not exceed 6 minutes, as color fading may occur, leading to inaccurate results.
- Medição: Insira o s preparadoample cell into your calibrated colorimeter. Follow your colorimeter's instructions to initiate the measurement. The colorimeter will display the free chlorine concentration in mg/L.
- Resultados recordes: Record the measured free chlorine concentration.

Image: A single YSI DPD Free Chlorine Reagent Powder packet. This silver foil packet contains the pre-measured reagent powder for a single test. The text "VARIO.LIT Chlorine FREE-DPD" is visible on the packet, indicating its contents and purpose.
Manutenção e Armazenamento
Proper storage and handling are crucial for maintaining the integrity and effectiveness of the reagent powder:
- Armazenar: Store the reagent powder packs in their original packaging in a cool, dry place, away from direct sunlight and extreme temperatures. Do not store near strong acids or bases.
- Prazo de validade: Refer to the expiration date printed on the packaging. Do not use expired reagents, as they may yield inaccurate results.
- Disposição: Dispose of used reagent and sample solutions according to local environmental regulations. Consult your local authorities for proper chemical waste disposal procedures.
- Limpeza: Always ensure sample cells and equipment are thoroughly cleaned after each use to prevent cross-contamination.
Solução de problemas
Se você encontrar problemas durante os testes, considere os seguintes problemas comuns e suas soluções:
| Problema | Possível causa | Solução |
|---|---|---|
| Sem desenvolvimento de cor | No free chlorine present; Expired reagent; Incorrect sampvolume. | Verificar sample contains chlorine; Check reagent expiration date; Ensure 5ml sampvolume. |
| Fading color or inaccurate reading | Reaction time exceeded; Contaminated sample cell; Turbidity in sampeu. | Adhere strictly to 1-minute reaction time; Clean sample cells thoroughly; Filter turbid sampse necessário. |
| Resultados inconsistentes | Improper mixing; Temperature variations; Colorimeter not calibrated. | Ensure complete dissolution of powder; Perform tests at consistent temperatures; Recalibrate colorimeter. |
Especificações
- Nome do produto: YSI DPD Free Chlorine Reagent Powder
- Número do modelo: 251398Y
- Reagent Type: DPD (N,N-Diethyl-p-phenylenediamine)
- Analito: Cloro Livre (Cl2)
- SampVolume: 5 ml
- Faixa de medição: 0 a 3.5 mg/L (ppm)
- Embalagem: Powder packs, 100 per pack
- Fabricante: YSI
- ASIN: B00CMX0PDK
- Dimensões da embalagem: 8.1 x 6.9 x 1.2 polegadas
- Peso do item: 3.2 onças
Garantia e Suporte
For information regarding product warranty, technical support, or to purchase additional reagents, please contact YSI customer service or visit the official YSI website. Consulte a documentação de compra para obter os termos e condições específicos da garantia.
YSI Contact Information:
- Website: www.ysi.com (Please note: This is a placeholder link, refer to official YSI documentation for exact contact details.)